- Exit-of-mitosis Mutants
- cdc14-ts
- cdc14-ts GAL-SIC1
- cdc14ts then GAL-SIC1
- cdc14-ts cdh1∆
- cdc14-ts sic1∆
- cdc14-ts GAL-CLN2
- net1-ts
- net1-ts in nocodazole
- net1∆ cdc20-ts
- tem1∆ net1-ts
- cdc15∆ net1-ts
- cdc15∆ net1-ts cdh1∆
- GAL-NET1
- GAL-CDC14
- GAL-NET1 GAL-CDC14
- cdc15-ts multicopy CDC14
- tem1-ts multicopy CDC14
- TAB6-1
- TAB6-1 cdc15∆
- TAB6-1 clb5∆ clb6∆
- TAB6-1 CLB1 clb2∆
net1∆ cdc20-ts
debug: ,
test user =
test db =
Simulation:
Change of parameters: kasrent=1, kasrentp=0.005, ks20'=ks20"=0.
Arrest: Mitotic exit at t=196 min with sister chromatids still attached.
Experiments:
Visintin, R., Hwang, E.S., Amon, A. (1999). Cfi1 prevents premature exit from mitosis by anchoring Cdc14 phosphatase in the nucleolus. Nature 398:818-823.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Table 1, inviable.
Comments: Sister chromatids do not separate (Pds1 is high at exit of mitosis).